RESUMO
Salmonella enteric serovar Typhimurium is the most common enteric pathogen in humans and animals. Consumption of contaminated food or water triggers inflammation that allows Salmonella to spread into the gut and causes gastrointestinal diseases. The infection spreads by intestinal invasion, phagocyte internalization and subsequent dissemination in many other patients. This research used TolA, a Salmonella typhimurium membrane protein, to computationally design a multi-epitope vaccine against the pathogen. Complete consistency of the candidate vaccine was checked In silico, and molecular dynamics simulations confirmed the vaccine's stability. According to docking report, the vaccine has a good affinity with toll-like receptors. In silico cloning and codon optimization techniques improved the vaccine's efficacy in Salmonella typhimurium manifestation process. The candidate vaccine induced an efficient immune response, as determined by In silico immune simulation. Computational studies revealed that the engineered multi-epitope vaccine is structurally stable, capable of eliciting particular immunological reactions, and therefore a candidate for a latent Salmonella typhimurium vaccine. However, wet lab studies and further investigations are required to confirm the results.
RESUMO
In this study hepatoprotective aptitude of Brachychiton populneus against carbon tetrachloride (CCl4) instigated liver injuries in rats was investigated. High-performance liquid chromatography (HPLC) with a diode array detector (DAD) analysis of methanol extract of B. populneus (BPM) indicated existence of rutin, catechin and myricetin. Administration of CCl4 to rat decreased (p < 0.01) the level of catalase (CAT), total superoxide dismutase (SOD), peroxidase (POD), soluble protein and reduced glutathione (GSH) whereas elevated the concentration of H2O2, thiobarbituric acid reactive substances and nitrite in hepatic samples. In serum the level of hepatic markers; aspartate transaminase, alanine transaminase, alkaline phosphatase and total bilirubin increased with CCl4 treatment against control animals. In hepatic samples the expression level of endoplasmic reticulum stress associated genes like glucose regulated protein (GRP78), x-box binding protein- 1 total (XBP-1 t), x-box binding protein- 1 spliced (XBP-1 s), x-box binding protein- 1 unspliced (XBP-1 u), glutamate-cysteine ligase catalytic subunit (GCLC) and pro-inflammatory cytokines; tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) was elevated many fold with CCl4 administration to rat. Co-administration of BPM along with CCl4 to rats decreased (p < 0.05) the expression of above genes except GCLC where expression level was enhanced as compared to CCl4 treatment. Histopathology of liver showed injuries of hepatocytes, infiltration of leukocytes and damaged central lobule in CCl4 treated rats. However, BPM administration to CCl4 intoxicated rats restored the altered parameters towards the control rats. These results suggested the presence of antioxidant and anti-inflammatory constituents in methanol extract of B. populneus.
